A Comparative Study of Cryo and Paraffin Sections in Localizing Gastrin Cells (g-cells) in the Stomach of Rana Cyanophlyctis (schneider) Using Immunocyto- Chemical Technique

The gastrointestinal tract is the first physiological state of animals involved in the transformation of diet into nutrients and their absorption across intestinal mucosa, the phenomenon of digestion. This entire episode is regulated by the cumulative actions of two unique components of alimentary canal i.e., enteric nervous and endocrine components (Guyton and Hall, 2008). The enteric endocrinocytes among vertebrates exist as diffused cells amidst mucosal cell population (Pearse, 1981). It is supposed that more than hundred regulatory substances including both hormones and putative hormones are secreted by the gastrointestinal mucosa (Hadley and Levine, 2009), hence gastrointestinal tract is also called as ‘largest endocrine gland in the body’ (Thompson et al., 1987; Ahlman and Nilsson, 2001).